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University Imaging Centers - Where the University sees the future

Journal Articles

Elena Goun

Nature Methods - 13 May 2019 - 12:00am

Nature Methods, Published online: 13 May 2019; doi:10.1038/s41592-019-0429-4

Bioluminescence lights her way to measure glucose uptake in vivo, and why a chemist travels outside her comfort zone.
Categories: Journal Articles

Bioluminescent-based imaging and quantification of glucose uptake in vivo

Nature Methods - 13 May 2019 - 12:00am

Nature Methods, Published online: 13 May 2019; doi:10.1038/s41592-019-0421-z

A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo.
Categories: Journal Articles

Light-sheet microscopy in the near-infrared II window

Nature Methods - 13 May 2019 - 12:00am

Nature Methods, Published online: 13 May 2019; doi:10.1038/s41592-019-0398-7

Light-sheet microscopy in the NIR-II window enables rapid volumetric imaging of tissues at impressive depths in vivo without invasive preparations owing to the reduced light scattering and tissue autofluorescence at these wavelengths.
Categories: Journal Articles

High-density multi-fiber photometry for studying large-scale brain circuit dynamics

Nature Methods - 13 May 2019 - 12:00am

Nature Methods, Published online: 13 May 2019; doi:10.1038/s41592-019-0400-4

High-density arrays of optical fibers enable monitoring and manipulation of neural activity at large scale across many brain regions. The multi-fiber arrays can be used in head-fixed tasks, in freely behaving animals and during social interactions.
Categories: Journal Articles

Software tools for automated transmission electron microscopy

Nature Methods - 13 May 2019 - 12:00am

Nature Methods, Published online: 13 May 2019; doi:10.1038/s41592-019-0396-9

Py-EM and SerialEM enable automated microscope control for high-throughput data acquisition in diverse transmission electron microscopy imaging experiments.
Categories: Journal Articles

Machine learning, practically speaking

Nature Methods - 9 May 2019 - 12:00am

Nature Methods, Published online: 09 May 2019; doi:10.1038/s41592-019-0432-9

To apply machine learning, labs needn’t have years of computational expertise, but they do need a cautious mind-set.
Categories: Journal Articles

Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

Nature Methods - 6 May 2019 - 12:00am

Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution

Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution, Published online: 06 May 2019; doi:10.1038/s41592-019-0401-3

Epi-illumination SPIM enables fast, volumetric, high-resolution, subcellular imaging of any sample compatible with a standard inverted fluorescence microscope.
Categories: Journal Articles

Polarization-varying anisotropic terahertz microscopy

Nature Methods - 30 April 2019 - 12:00am

Polarization-varying anisotropic terahertz microscopy

Polarization-varying anisotropic terahertz microscopy, Published online: 30 April 2019; doi:10.1038/s41592-019-0419-6

Polarization-varying anisotropic terahertz microscopy
Categories: Journal Articles

Optically mapping methylation

Nature Methods - 30 April 2019 - 12:00am

Optically mapping methylation

Optically mapping methylation, Published online: 30 April 2019; doi:10.1038/s41592-019-0420-0

Optically mapping methylation
Categories: Journal Articles

Diet influences microbe–host interaction

Nature Methods - 30 April 2019 - 12:00am

Diet influences microbe–host interaction

Diet influences microbe–host interaction, Published online: 30 April 2019; doi:10.1038/s41592-019-0413-z

Diet influences microbe–host interaction
Categories: Journal Articles

A bacterial treasure trove

Nature Methods - 30 April 2019 - 12:00am

A bacterial treasure trove

A bacterial treasure trove, Published online: 30 April 2019; doi:10.1038/s41592-019-0411-1

A newly identified bacterial biosynthetic pathway enables the production of nonstandard amino acids.
Categories: Journal Articles

Bright probes for EM

Nature Methods - 30 April 2019 - 12:00am

Bright probes for EM

Bright probes for EM, Published online: 30 April 2019; doi:10.1038/s41592-019-0417-8

Bright probes for EM
Categories: Journal Articles

All the right moves

Nature Methods - 30 April 2019 - 12:00am

All the right moves

All the right moves, Published online: 30 April 2019; doi:10.1038/s41592-019-0424-9

Dispelling stigmas and highlighting benefits of the Nature Research manuscript transfer service.
Categories: Journal Articles

Bypassing bleaching with fluxional fluorophores

Nature Methods - 30 April 2019 - 12:00am

Bypassing bleaching with fluxional fluorophores

Bypassing bleaching with fluxional fluorophores, Published online: 30 April 2019; doi:10.1038/s41592-019-0402-2

Combining photoactivation and spontaneous blinking enables long time-lapse localization microscopy.
Categories: Journal Articles

Imaging chromatin and RNA in embryos

Nature Methods - 30 April 2019 - 12:00am

Imaging chromatin and RNA in embryos

Imaging chromatin and RNA in embryos, Published online: 30 April 2019; doi:10.1038/s41592-019-0414-y

Imaging chromatin and RNA in embryos
Categories: Journal Articles

Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads

Nature Methods - 22 April 2019 - 12:00am

Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads

Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads, Published online: 22 April 2019; doi:10.1038/s41592-019-0394-y

Replication initiation is stochastic and obscured in population sequencing; D-NAscent reports the use of long nanopore reads to detect base analogs and thus to assess replication initiation at the individual molecule level.
Categories: Journal Articles

Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells

Nature Methods - 22 April 2019 - 12:00am

Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells

Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells, Published online: 22 April 2019; doi:10.1038/s41592-019-0392-0

ECCITE-seq combines the single-cell analysis of multiple modalities, for example transcriptome, immune cell receptors, cell surface proteins and single-guide RNAs.
Categories: Journal Articles

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Nature Methods - 22 April 2019 - 12:00am

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Engineered peptide barcodes for in-depth analyses of binding protein libraries, Published online: 22 April 2019; doi:10.1038/s41592-019-0389-8

A method for protein binder selection and identification, NestLink, uses barcoding peptides detectable by mass spectrometry to select and biophysically characterize thousands of binders without requiring the handling of individual clones.
Categories: Journal Articles

Publisher Correction: A cheminformatics approach to characterize metabolomes in stable-isotope-labeled organisms

Nature Methods - 16 April 2019 - 12:00am

Publisher Correction: A cheminformatics approach to characterize metabolomes in stable-isotope-labeled organisms

Publisher Correction: A cheminformatics approach to characterize metabolomes in stable-isotope-labeled organisms, Published online: 16 April 2019; doi:10.1038/s41592-019-0423-x

Publisher Correction: A cheminformatics approach to characterize metabolomes in stable-isotope-labeled organisms
Categories: Journal Articles

A photocleavable surfactant for top-down proteomics

Nature Methods - 15 April 2019 - 12:00am

A photocleavable surfactant for top-down proteomics

A photocleavable surfactant for top-down proteomics, Published online: 15 April 2019; doi:10.1038/s41592-019-0391-1

A mass-spectrometry-compatible surfactant called Azo effectively solubilizes proteins, is rapidly degraded by ultraviolet irradiation and enables top-down proteomic analysis of membrane proteins.
Categories: Journal Articles

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