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Journal Articles

The politically engaged scientist

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 457 (2017). doi:10.1038/nmeth.4289

The appetite for political engagement among scientists across the United States has increased since the 2016 election. If well channeled and sustained, this would be a positive development that could last beyond the current administration's tenure.

Categories: Journal Articles

The Author File: Koraljka Husnjak

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 459 (2017). doi:10.1038/nmeth.4266

Author: Vivien Marx

A “simple but a bit crazy idea” to tag ubiquitin and practice multilingual, multidisciplinary proteomics.

Categories: Journal Articles

Systems biology guided by XCMS Online metabolomics

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 461 (2017). doi:10.1038/nmeth.4260

Authors: Tao Huan, Erica M Forsberg, Duane Rinehart, Caroline H Johnson, Julijana Ivanisevic, H Paul Benton, Mingliang Fang, Aries Aisporna, Brian Hilmers, Farris L Poole, Michael P Thorgersen, Michael W W Adams, Gregory Krantz, Matthew W Fields, Paul D Robbins, Laura J Niedernhofer, Trey Ideker, Erica L Majumder, Judy D Wall, Nicholas J W Rattray, Royston Goodacre, Luke L Lairson & Gary Siuzdak

Categories: Journal Articles

Addressing reproducibility in single-laboratory phenotyping experiments

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 462 (2017). doi:10.1038/nmeth.4259

Authors: Neri Kafkafi, Ilan Golani, Iman Jaljuli, Hugh Morgan, Tal Sarig, Hanno Würbel, Shay Yaacoby & Yoav Benjamini

Categories: Journal Articles

Bioinformatics: Defining developmental grammar

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 465 (2017). doi:10.1038/nmeth.4279

Author: Nicole Rusk

An iterative clustering approach finds the few genes that mark discrete cell states and their transitions during early mouse development.

Categories: Journal Articles

Stem cells: It takes two to make an embryo

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 466 (2017). doi:10.1038/nmeth.4280

Author: Irene Jarchum

In vitro culture of mouse embryonic and extra-embryonic stem cells recapitulates embryogenesis.

Categories: Journal Articles

Cell biology: A light switch for kinases

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 466 (2017). doi:10.1038/nmeth.4281

Author: Nina Vogt

A photodissociable dimer of the Dronpa fluorescent protein can cage kinases, making these important signal transducers controllable by light.

Categories: Journal Articles

Proteomics: A proteome-wide view of thermal stability

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 468 (2017). doi:10.1038/nmeth.4282

Author: Allison Doerr

Proteome thermal stability is probed using limited proteolysis and mass spectrometry.

Categories: Journal Articles

Imaging: Raman imaging in 3D cultures

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 469 (2017). doi:10.1038/nmeth.4269

Categories: Journal Articles

Genomics: Human lncRNA atlas

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 469 (2017). doi:10.1038/nmeth.4270

Categories: Journal Articles

Nanobiotechnology: Measuring single-molecule charges

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 469 (2017). doi:10.1038/nmeth.4271

Categories: Journal Articles

Synthethic biology: A fully designed yeast genome

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 469 (2017). doi:10.1038/nmeth.4272

Categories: Journal Articles

Biochemistry: Capturing ubiquitin-binding interactions

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 470 (2017). doi:10.1038/nmeth.4273

Categories: Journal Articles

Neuroscience: Light-dependent inhibition of CaMKII

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 470 (2017). doi:10.1038/nmeth.4276

Categories: Journal Articles

Sensors and probes: Dynamic measurement of membrane charges

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 471 (2017). doi:10.1038/nmeth.4283

Author: Rita Strack

A FRET sensor enables quantitative characterization of electrostatic potential of membranes in living cells.

Categories: Journal Articles

How to deduplicate PCR

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 473 (2017). doi:10.1038/nmeth.4268

Author: Vivien Marx

PCR duplicates—sequencing reads from the same original genomic fragment—can cause headaches. But there are remedies.

Categories: Journal Articles

Human T cell development notched up a level

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 477 (2017). doi:10.1038/nmeth.4277

Authors: Anne-Catherine Dolens & Tom Taghon

Two complementary approaches for directing human hematopoietic stem cells along the T cell lineage will have applications in both fundamental and translational research.

Categories: Journal Articles

Corrigendum: Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry

Nature Methods - 27 April 2017 - 12:00am

Nature Methods 14, 539 (2017). doi:10.1038/nmeth0517-539

Author: Fan Liu, Dirk T S Rijkers, Harm Post & Albert J R Heck

Categories: Journal Articles

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