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University Imaging Centers - Where the University sees the future

Journal Articles

Genome-wide C-SWAT library for high-throughput yeast genome tagging

Nature Methods - 9 July 2018 - 12:00am

Genome-wide C-SWAT library for high-throughput yeast genome tagging

Genome-wide C-SWAT library for high-throughput yeast genome tagging, Published online: 09 July 2018; doi:10.1038/s41592-018-0045-8

A C-SWAT acceptor library allows the user to easily insert any tag of choice after yeast ORFs by swapping it for the acceptor module.
Categories: Journal Articles

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Nature Methods - 9 July 2018 - 12:00am

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy, Published online: 09 July 2018; doi:10.1038/s41592-018-0052-9

Bright reversibly switching red fluorescent proteins (rsFusionReds) with fast switching kinetics and low fatigue enable RESOLFT and MoNaLISA nanoscopy of live cells with green-orange illumination, which further reduces the risk of phototoxicity.
Categories: Journal Articles

Genome-wide SWAp-Tag yeast libraries for proteome exploration

Nature Methods - 9 July 2018 - 12:00am

Genome-wide SWAp-Tag yeast libraries for proteome exploration

Genome-wide SWAp-Tag yeast libraries for proteome exploration, Published online: 09 July 2018; doi:10.1038/s41592-018-0044-9

A genome-wide collection of N-terminally tagged yeast libraries allows easy swapping of tags and exploration of the yeast proteome.
Categories: Journal Articles

Targeting Cas9

Nature Methods - 2 July 2018 - 12:00am

Targeting Cas9

Targeting Cas9, Published online: 02 July 2018; doi:10.1038/s41592-018-0061-8

Targeting Cas9
Categories: Journal Articles

Inferring neuronal activity from gene expression

Nature Methods - 2 July 2018 - 12:00am

Inferring neuronal activity from gene expression

Inferring neuronal activity from gene expression, Published online: 02 July 2018; doi:10.1038/s41592-018-0064-5

Inferring neuronal activity from gene expression
Categories: Journal Articles

Metastasis in a dish

Nature Methods - 2 July 2018 - 12:00am

Metastasis in a dish

Metastasis in a dish, Published online: 02 July 2018; doi:10.1038/s41592-018-0067-2

Metastasis in a dish
Categories: Journal Articles

StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins

Nature Methods - 2 July 2018 - 12:00am

StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins

StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins, Published online: 02 July 2018; doi:10.1038/s41592-018-0041-z

Stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease enable robust, reversible, and orthogonal chemogenetic control of protein functions, including CRISPR–Cas9 activity, transcription, and protein dimerization.
Categories: Journal Articles

Correlating behavior and neural activity at high resolution

Nature Methods - 2 July 2018 - 12:00am

Correlating behavior and neural activity at high resolution

Correlating behavior and neural activity at high resolution, Published online: 02 July 2018; doi:10.1038/s41592-018-0057-4

A combination of behavioral analysis and neural activity recording provides a glimpse into how the brain orchestrates complex behaviors.
Categories: Journal Articles

Chemogenetic control of gene expression and cell signaling with antiviral drugs

Nature Methods - 2 July 2018 - 12:00am

Chemogenetic control of gene expression and cell signaling with antiviral drugs

Chemogenetic control of gene expression and cell signaling with antiviral drugs, Published online: 02 July 2018; doi:10.1038/s41592-018-0042-y

Ligand-inducible connection (LInC) combines HCV protease and protease inhibitors to offer an orthogonal chemogenetic approach for controlling protein functions including protein localization, gene expression, and cell–cell communication.
Categories: Journal Articles

Transcripts from a spliceosome

Nature Methods - 2 July 2018 - 12:00am

Transcripts from a spliceosome

Transcripts from a spliceosome, Published online: 02 July 2018; doi:10.1038/s41592-018-0058-3

Two new methods provide high-resolution profiles of splicing events in yeast.
Categories: Journal Articles

Bioconda: sustainable and comprehensive software distribution for the life sciences

Nature Methods - 2 July 2018 - 12:00am

Bioconda: sustainable and comprehensive software distribution for the life sciences

Bioconda: sustainable and comprehensive software distribution for the life sciences, Published online: 02 July 2018; doi:10.1038/s41592-018-0046-7

Bioconda: sustainable and comprehensive software distribution for the life sciences
Categories: Journal Articles

US universities go East

Nature Methods - 2 July 2018 - 12:00am

US universities go East

US universities go East, Published online: 02 July 2018; doi:10.1038/s41592-018-0060-9

The global expansion of Western universities is not without challenges but can provide manifold opportunities, particularly in the Middle East.
Categories: Journal Articles

Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs

Nature Methods - 2 July 2018 - 12:00am

Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs

Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs, Published online: 02 July 2018; doi:10.1038/s41592-018-0017-z

Fusion of a guide RNA directly to an adenosine deaminase via a SNAP-tag allows for precise and efficient RNA editing.
Categories: Journal Articles

Controlling protein function with HCV protease

Nature Methods - 2 July 2018 - 12:00am

Controlling protein function with HCV protease

Controlling protein function with HCV protease, Published online: 02 July 2018; doi:10.1038/s41592-018-0043-x

Two methods for controlling protein activities with small molecules provide a general solution to a long-standing challenge in mammalian synthetic biology.
Categories: Journal Articles

A reassessment of DNA-immunoprecipitation-based genomic profiling

Nature Methods - 25 June 2018 - 12:00am

A reassessment of DNA-immunoprecipitation-based genomic profiling

A reassessment of DNA-immunoprecipitation-based genomic profiling, Published online: 25 June 2018; doi:10.1038/s41592-018-0038-7

Reanalysis of DNA-immunoprecipitation-based data shows that modification-specific antibodies bind unmodified short tandem repeats, and IgG controls are needed to avoid false positives.
Categories: Journal Articles

DeTiN: overcoming tumor-in-normal contamination

Nature Methods - 25 June 2018 - 12:00am

DeTiN: overcoming tumor-in-normal contamination

DeTiN: overcoming tumor-in-normal contamination, Published online: 25 June 2018; doi:10.1038/s41592-018-0036-9

DeTiN can identify and account for signal from tumor cells that contaminate normal samples, for more sensitive somatic variant detection.
Categories: Journal Articles

SAVER: gene expression recovery for single-cell RNA sequencing

Nature Methods - 25 June 2018 - 12:00am

SAVER: gene expression recovery for single-cell RNA sequencing

SAVER: gene expression recovery for single-cell RNA sequencing, Published online: 25 June 2018; doi:10.1038/s41592-018-0033-z

SAVER accurately recovers expression values in single-cell RNA-sequencing data to improve downstream analysis.
Categories: Journal Articles

GeNets: a unified web platform for network-based genomic analyses

Nature Methods - 18 June 2018 - 12:00am

GeNets: a unified web platform for network-based genomic analyses

GeNets: a unified web platform for network-based genomic analyses, Published online: 18 June 2018; doi:10.1038/s41592-018-0039-6

The GeNets web platform can identify the most informative network, as well as execute, store and share network-based analyses of RNA-seq or genomic datasets.
Categories: Journal Articles

EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

Nature Methods - 18 June 2018 - 12:00am

EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification, Published online: 18 June 2018; doi:10.1038/s41592-018-0037-8

EASI-tag, a new type of isobaric labeling reagents, enables multiplexed and highly accurate proteome quantification by mass spectrometry.
Categories: Journal Articles

A comparison of methods to assess cell mechanical properties

Nature Methods - 18 June 2018 - 12:00am

A comparison of methods to assess cell mechanical properties

A comparison of methods to assess cell mechanical properties, Published online: 18 June 2018; doi:10.1038/s41592-018-0015-1

This Analysis compares and contrasts methods for measuring the mechanical properties of cells by applying the different approaches to the same breast cancer cell line.
Categories: Journal Articles

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