University of Minnesota
University Imaging Center
http://uic.umn.edu/
612.624.7938
University Imaging Centers - Where the University sees the future

Equipment Overview

Equipment Capabilities Location

Amersham Imager 600UV

The Amersham Imager 600UV is a sensitive and robust imager for chemifluorescence, UV transillumination, and UV fluorescence analysis of protein and DNA samples in gels and membranes.

Room 1-164 Jackson Hall
Reserve Equipment
,

Bruker Xtreme Optical and X-ray small animal imaging system

ATTENTION: THIS SYSTEM IS LOCATED IN THE CANCER CARDIOVASCULAR RESEARCH BUILDING, RM 1-250E. PLEASE CONTACT UIC STAFF FOR ACCESS AND TRAINING BEFORE USING.

The Xtreme system combines high-sensitivity optical molecular imaging (Biolumenescence and Fluorescence) along with high-resolution digital x-ray to deliver precise anatomical localization of molecular and cellular biomarkers in vivo.  Software automation simplifies complex multimodal imaging protocols for small animal imaging. The system includes animal handling features such as a heated sample shelf, a gas anesthesia system and MARS 360 degree animal rotation system (https://www.youtube.com/watch?v=V49qHYAqWsY). 

Bone denstiy measurements are possible: https://www.youtube.com/watch?v=FPfd6_5rung

System purchased with the generous support of the OVPR, CBS and AHC/Medical School through an I3 award. 

•Please do not use this equipment without contacting UIC staff for training and approval. Request training here:

  http://uic.umn.edu/node/add/training-request

•Please return all animals to the Catch Room (rm 1-370F) following imaging.  Contact RAR staff for housing information.

•Please contact UIC staff with any questions.

  http://uic.umn.edu/

 

Room 1-250E CCRB
Reserve Equipment

Canon EOS 40D DSLR

The EOS 40D combines tremendous know-how in both the digital and photographic worlds, creating a camera that not only does everything one would expect of a traditional digital SLR, but one that incorporates staggering leaps forward in technological innovation. With new features like EOS Integrated Cleaning System, Live View Function, a more powerful DIGIC III Image Processor, plus a 10.1-megapixel CMOS sensor, and a 3.0-inch LCD monitor.

1-151 Jackson Hall (Minneapolis Campus)
Reserve Equipment

Canon EOS Rebel XT DSLR

Featuring Digital Trinity - an 8.0 Megapixel CMOS sensor, DIGIC II Image Processor and compatibility with over 50 EF Lenses - the new Digital Rebel XT has an all new lightweight and compact body, improved performance across the board and the easiest operation in its class, simplifying complex tasks and ensuring the perfect shot every time. 

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

CareStream Gel Pro 212 Imager

This versatile, easy-to-use system is ideal for imaging fluorescence or colorimetric data in sample formats such as gels (DNA/Coomassie/Silver stain) membranes, and 96 well plates. Carestream Molecular Imaging software (included) features a variety of new, easy-to-use functionalities such as true autofocus and directed autoexposure make it easy to capture perfectly exposed images in just a few clicks. The system is equipped with a thermal printer.

McGuire Translational Research Facility (Minneapolis Campus)
Reserve Equipment

Countess Automated Cell Counter

The Countess® Automated Cell Counter offers easy and accurate cell counting and viability counts. You can get all the data you need about your cell cultures in just 30 seconds without using a hemocytometer.

Room 1-220E CCRB
Reserve Equipment

Denton DV-502 Vacuum Evaporator

The Denton Vacuum Evaporator is used in many different specimen preparation methods — mostly employed in electron imaging. Thicker coatings of metal (such as gold, platinum:palladium, or gold:palladium) can be applied to specimens of nearly any shape.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Epson EMP-730 (Gray)

The Epson EMP-730 multimedia projector showcases Epson's technological breakthroughs, weighs in at just 1.9Kg and delivers 2000 ANSI Lumens brightness. Designed for professional presenters who require the ultimate audiovisual support either in the office or on the road, this mini powerhouse ensures stunning results in any environment.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

FlowCAM®

The FlowCAM® automatically captures digital images of each particle as it passes by in a fluid stream and records up to 26 different measurements for each particle.

Watch the video

430 Ecology, Evolution and Behavior (EEB)
Reserve Equipment

Fujitsu Tablet PC (Mr. Sparkle)

Fujitsu Tablet

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Fullam Sputter Coater

Fullam Sputter Coater

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Hitachi S3500N Variable Pressure SEM

The Hitachi S3500N Variable Pressure Scanning Electron Microscope (SEM). Quartz PCI digital imaging, Stage with motor driven X & Y axes, Environmental Secondary Electron Detector (ESED), Absorbed Electron EBIC detector, Robinson Backscattered Electron Detector (BSE), standard Secondary Electron Detector (SED), Infrared ChamberScope, EDAX Phoenix x-ray microanalysis system with Super Ultra-thin window, 10mm Compact Detector Unit, Windows NT Operating System, elemental mapping, spectral processing, multi-point analysis, Hitachi Hi-Mouse software, Emitech K-1150 Cryogenic System, with cryo-prep unit, airlock interface to SEM, sputter coater.

Room 37D Snyder Hall
Reserve Equipment

HP Mini Netbook (Otto)

An ultra-lightweight netbook you can take everywhere to stay connected.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Huron Technologies TISSUEscope LE

The UIC provides color brightfield slide scanning as a service at $15/slide.  The TissueScope LE is a brightfiled slide scanner that can scan up to twelve (12) 1x3" or (6) 2x3" slides at a time as save the entire slide image in .tif format.  The scans can be done at 20x and 40x of the entire tissue section on the slide. 

The slides can be dropped at any UIC location (the scanner is in 1-220 CCRB) and then picked up in 1-220 CCRB once scanned.  The turnaround time is dependent on the number of slides with an average of a 12-24 slides per day scan rate. There service is requested from https://my.ilabsolutions.com/service_center/2948?tab=services.

Please be sure the slides are clean, free from any excess mounting media and ready to image.

 

Room 1-220A CCRB
Reserve Equipment

Image Analysis & Deconvolution Workstation

Computer Workstation

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Imaris Workstation - Jackson Hall

Imaris is a commercial software package allowing to perform advanced and challenging interactive multi-dimentional image analyses. Due to the high hardware requirements the UIC has purchased a high-performance image processing workstation (Win7 64 bit, 24 Gb RAM, 2Gb graphics card, several harddisks,etc...) located inroom 1-153 Jackson Hall. In general the software is mainly popular for 3D analysis of confocal images, isosurface renderings and advanced movies.

Room 1-153 Jackson Hall
Reserve Equipment

Infrared SnapShot Thermography Camera

The Infrared Solutions SnapShot camera is a precision portable uncooled infrared imaging radiometer. The thermoelectric sensor is inherently radiometric and exhibits excellent dynamic range. Accurate temperature measurement combined with low cost, make the IR SnapShot ideal for cost-sensitive applications such as predictive and preventive maintenance, quality control and new product development. The intuitive user interface and controls make learning and using this revolutionary camera a snap.

1-151 Jackson Hall (Minneapolis Campus)
Reserve Equipment

IVIS Spectrum In Vivo Imaging System

The IVIS Spectrum is the most versatile and advanced in vivo imaging system available on the market today. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Additional details, specifications and vendor protocols can be found here: http://www.perkinelmer.com/Catalog/Product/ID/IVISSPE

 

Room 1-166A Jackson Hall
Reserve Equipment

Leica AFS2 Freeze Substitution and Low Temperature Embedding System

The Leica EM AFS2 performs freeze substitution and progressive lowering temperature (PLT) techniques and allows low temperature embedding and polymerization of resins.
Room 37A Snyder Hall
Reserve Equipment

Leica CM1800 Cryostat

Leica CM 1800 Cryostat utilizes up-to date microprocessor technology ensuring easy operation. All functions are protected against dirt and humidity by a plastic foil. It features an easily readable display including an automatic display of errors and a motorized coarse feed which enables rapid trimming and sectioning.

Room 37A Snyder Hall
Reserve Equipment

Leica EM Tissue Processor

Tissues can be processed into a variety of resins for subsequent electron and light microscopy analysis. The pre-heat/cool system allows the correct reagent temperature to be maintained throughout processing. Small vials are available for EM processing and larger for LM which can also be used for high throughput sample processing runs where over 150 EM samples can be processed in one run.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Leica MZ FL III Fluorescence Stereomicroscope

Sort and manipulate and image your specimens with the stereo objective in 3D, with large fields of view, large working distances and an outstanding depth of field. Simply switch to the HR (High Resolution) micro-objective and analyze the quality of the gene expression immediately and without delay via an optical light microscope at a resolution of 0.7 µm (1320 pairs of lines/mm) and an 800x magnification.

Room 1-250D CCRB
Reserve Equipment
,

Leica SP5 Multiphoton Confocal Upright Microscope

Leica DM6000 CFS upright multiphoton microscope equipped with a TCS SP5 MP laser confocal with both galvonomic and resonance scanners and HyD detectors. 405, 457, 488, 561, and 640 nm single photon laser lines and a Mai Tai eHP DS 690-1040 nm tunable multiphoton laser. Objectives available include 63x/NA 1.2 W with correction collar and 25x/NA 0.95 water with correction collar (optimal for intravital deep tissue imaging (up to 1 mm)). Additional objectives include: Plan Apo 10x/0.40; Plan Apo 20x/0.70 with correction collar; IRApo 25x/0.95 Water; Plan Apo 40x/1.30 oil immersion; Plan Apo 63x/1.4-0.6 oil immersion. 

Room 1-250D CCRB
Reserve Equipment

Leica Ultracut UCT Ultramicrotome

The ultramicrotome is an instrument commonly used to prepare TEM samples. It uses either a glass or diamond knife to cut ultra thin sections of specimens embedded in epoxy. The sections are approximately 60 to 100 nanometers in thickness and 0.5 to 3 mm in width and can be stained and mounted onto TEM grids for viewing. The ultramicrotome is also used to create mirror-smooth surfaces on samples mounted in epoxy, the applications for which include secondary imaging on the SEM and nanoindentation. Preparation on smooth samples for surface analysis need not always be of samples that are embedded, as sometimes bulk will work.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment
, ,

LMD6500 Laser Dissection Microscope

Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected for subsequent molecular analysis.

Researchers can selectively and routinely analyze regions of interest down to single cells from all kinds of tissues (cryo or paraffin sections 4-20 µm thick), even living cells from cell culture.  

Check out this training video using the LMD6500 on JOVE: http://www.jove.com/video/51168/production-rna-for-transcriptomic-analys...

You have the abiltiy to de-paraffinize your slides on site.

LDM works best when using PEN membrane slides.  The membrane only fram slides are available in the LifeTechnolgy Supply Center on site or you can order them here: http://www.lifetechnologies.com/order/catalog/product/LCM0521?ICID=searc...

The PEN membrane on glass slides are here:https://www.thermofisher.com/order/catalog/product/LCM0522?ICID=search-p...

 

Videos on the LDM technique can be found here: http://www.thermofisher.com/us/en/home/life-science/gene-expression-anal...

Room 1-220B CCRB
Reserve Equipment

M400 Tablet PC (Milhouse)

Toshiba's third generation Tablet PC.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Maestro In-Vivo Imaging System

Maestro provides users with a patented method of removing autofluorescence emitted from images of plant and animal tissues to reveal otherwise hard-to-detect labeled targets of interest. The dramatic improvement in signal-to-noise can increase sensitivity up to several hundred-fold, enabling much smaller or fainter signals from biological targets to be detected earlier and accurately measured. The increased sensitivity over standard imaging techniques provided by Maestro’s high-quality spectral imaging technology enable experiments and models which simply cannot be performed on any other system.

Room 37A Snyder Hall
Reserve Equipment

Nikon A1 Spectral Confocal Microscope

The Nikon A1si is a confocal system equipped with a point-scan scan head, 5 standard PMT detectors and a 32-channel PMT spectral detector. The A1si's highest resolution mode is up to 4096 x 4096 pixels. The system is mounted on a Nikon Ti2000E inverted, fluorescence microscope with DIC optics. NIS Elements imaging software is used to control acquisition and analysis.

Advantages of multispectral imaging and unmixing has been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging. 

A few example citations are:

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging "Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct;Chapter 14:Unit 14.19. doi: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.

Room 35D Snyder Hall
Reserve Equipment

Nikon A1R GaAsP Confocal Microscope

LIVE CELL IMAGING

Nikon Ti-E Motorized Microscope: Features include Nikon's patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 20x water immersion, 40x oil, and 60x oil magnifications for DIC. Fluorescence illumination and filters for widefield applications for DAPI, CFP, GFP, YFP, RFP, and Cy5. The scope is equipped for widefield imaging using a Hamamatsu Flash 4 V3 CMOS camera.

  • A1R GaAsP Confocal System
  • Laser Selection: 405nm, 445nm, 488nm, 514nm, 561nm and 640nm.
  • AR1 Confocal Scanner: Nikon's exclusive hybrid scanner allows flexible switching or simultaneous use of two galvo scanners (resonant and non-resonant) this allows ultrafast imaging (up to 420fps) and photo activation imaging required to unveil cell dynamics and interaction.
  • Detectors: Included are 4 confocal detectors (2 GaSaP) plus transmitted detector.

The Picoquant Fluorescence Lifetime Imaging (FLIM) system has been installed and is operational.  It has 405, 485 and 560 nm pulsed laser and cand do many FLIM and FCS techniques. Please contact UIC staff for training.

Learn more about FLIM here: http://tcspc.com/doku.php  ath the 

Time Correlated Single Photon Counting Wiki

 

 

Room 1-220H CCRB
Reserve Equipment
, ,

Nikon A1RMP Confocal

Nikon A1RMP confocal is connected to an upright Nikon FN1 microscope featuring a Plan Apo LWD 25x water-immersion, NA 1.1 objective most suitable for imaging deep (it has a 2mm working distance) into a sample. In addition to conventional sample confocal imaging, a Spectra Physics 15W Mai Tai eHP tunable IR laser and 4 non-descanned detectors allow multiphoton visualization of multiple fluorophores located deep within a living specimen, including 405, 457, 488, 514, 561 and 637 nm laser lines for imaging/photoactivation/photoconversion. The system has both galvonomic and resonance scanners that can be used in concert simultaneously for photoactivation and MP imaging.   and the enhanced hybrid PMTs. An ASI piezo motorized stage can be used for tiling or multiple-position imaging. The system is located in 1-166B Jackson Hall.  The room is equipped with a 5’ BSC cabinet and anesthesia station designed for tissue and whole animal imaging.

The system is equipped with Second Harmonic Generation (SHG) detection.  SHG doesn't involve the excitation of molecules like other techniques such as fluorescence microscopy. See UIC staff for training and questions.

 

This instrument was funded in 2012 by the Minnesota Partnership in conjuction with the Mayo Clinic Foundation, Rochester, MN.

Room 1-166B Jackson Hall
Reserve Equipment
, ,

Nikon A1Rsi Confocal w/ SIM Super Resolution

LIVE CELL IMAGING

 

Nikon Ti-E Motorized Microscope: Features include Nikon’s patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 10x, 20x, 40x, 60x, and 100x magnifications for DIC. Fluorescence accessories for widefield applications for DAPI, GFP, RFP, and Cy5.

·      A1R Confocal System

·      Laser Selection

405nm, 488nm, 561nm and 640nm

·       A1R Scanner- Nikon's exclusive hybrid scanner allows flexible switching or simultaneous use of two galvo scanners (resonant and non-resonant) this allows ultrafast imaging and photo activation imaging required to unveil cell dynamics and interaction.

·      Detectors- Included are 4 channel GaSP detectors, transmitted, and 32 channel Si (Spectral) detectors.

·      SIM Structured Illumination System-Using high frequency Structured Illumination, the Nikon N-SIM can achieve image resolution of 85nm*, which was previously considered impossible with optical microscopes. Furthermore, with temporal resolution of up to 0.6 sec/frame**, N-SIM enables super resolution time-lapse imaging capture of dynamic molecular interactions in living cells. Learn more about the product here: http://www.nikoninstruments.com/Products/Microscope-Systems/Inverted-Microscopes/N-SIM-Super-Resolution

·      Laser Selection

405nm, 488nm, 561nm and 640nm

·      A1/SIM Camera, Software and Workstation

Elements software and an Andor iXon camera complete the system and provide an easy to use customizable user interface to make the Nikon A1R/SIM system a turnkey live cell imaging system with multiple capabilities. 

Room 1-159 Jackson Hall
Reserve Equipment
,

Nikon AZ100 C1si Spectral Confocal Macroscope

New confocal imaging system for high-definition macro acquisition.

Our AZ100 Multizoom Macroscope with the C1si Spectral Confocal attachment, make the ultimate platform for in vivo imaging. Ideal for plant biology, developmental biology, cell biology, stem cell and tissue research, the AZ-C1si allows the researcher to view large specimens in confocal mode. The AZ-C1si can not only capture fields of view up to 1cm and permits deeper confocal imaging than conventional microscopes thanks to its large working distance objectives. Whole organisms can be monitored and documented over time (for example, embryos) offering a wealth of continuous information on development or the organism’s response to experimental variables. Both conventional PMTs and Spectral detectors are installed on this instrument.

The system is equipped with 1x, 4x and 5x objectives with variable optical zoom.  There are 405, 457, 476, 488, 514, 561, 637 nm lasers for image, co-localization, FRAP, FRET and spectral studies.

The system is run using Nikon Elements Software.

Advantages of multispectral imaging and unmixing has been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging. 

A few example citations are:

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging "Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct;Chapter 14:Unit 14.19. doi: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment
, ,

Nikon AZ100M Macro Fluorescence Scope

 

Nikon AZ100 Motorized Fluorescence Macroscope

  • Fluorescence (UV, CFP, GFP, YFP, dsRed and CY5 fliter sets ) and transmitted light with DIC
  • 1x and 4x PlanApo objectives
  • 1X to 8X Optical Zoom Range
  • Photometrics ES2 greyscale 12-bit camera for fluorescence
  • Nikon DS-Ri1 color 12-bit camera up to 4076x3116 pixels for full color imaging
  • Motorized X/Y stage for multi-position or panoramic images
  • Motorized Z for z-series collections for 3D and Extended Depth of Field Imaging
  • Nikon Elements AR software for multi-dimentional data collection

 

Room 1-161 Jackson Hall
Reserve Equipment

Nikon BioStation IM Live Cell Incubator and Imaging in Jackson and CCRB locations

LIVE CELL IMAGING

The Nikon BioStation IM is a compact live cell incubator and imaging system that allows users to conduct live cell imaging without a steep learning curve. The BioStation IM facilitates long term time-lapse experiments, including studies of cell growth, morphology, and protein expression, by providing consistent environmental control of temperature, humidity and gas concentration in combination with phase and fluorescence imaging (blue and green excitation) of exceptional quality. Multiple postions and z-series can be collected at various magnifications from 20x to 80x (0.8na). Additional fluorescence filters sets for UV, CYP, YFP and CY5 are available, with any two possible in each experiment.

There are Nikon Biostations located in both our Jackson Hall and CCRB locations.

The systems are best used with polymer or glass-bottomed 35mm dishes. 

Inserts are available to allow for multiple samples per experiment.

Room 1-161 Jackson Hall
Reserve Equipment

Nikon D70 SLR Digital Camera

6.1 Effective Megapixel Nikon DX Format CCD: Optimized to deliver a wide dynamic range that produces great pictures with high resolution and sharp details, the DX Format CCD sensor's 6.1 effective megapixels yield 3,008 x 2,000-pixel images that are suitable for making large prints, or for creative cropping to bring out detail.

1-151 Jackson Hall (Minneapolis Campus)
Reserve Equipment

Nikon Eclipse 90i

 

The Nikon Eclipse 90i is a fully motorized upright microscope configured to image in transmitted light, DIC and epi-fluorescence modes. Fluorescence cubes available for DAPI, GFP, RFP, and HYQ Cy5. Fluorescence illumination is provided by a Lumencor Sola Light Engine. You can do timelapse, XYZ position, Z stacks, multiple excitation or emission wavelengths, and tiling experiments. Equipped with a Hamamatsu Orca Flash 4.0 v2 CMOS monochrome camera and a Nikon D2-Fi2 color camera running on Nikon Elements software.

Room 23C Snyder Hall
Reserve Equipment
,

Nikon Eclipse TE2000-U Inverted Microscope

Nikon TE2000  Inverted Microscope

 

This Nikon TE2000 inverted microscope is equipped with brightfield, phase contrast and fluorescence capabilities.  It has a high sensitivity Nikon Ds-Fi1 color camera  (2560x1920 pixels) operated using Elements software.  

Fluorescence illumination is provided by a SOLA light engine or a Xenon Light source and the scope has filter sets for FURA, UV, CFP, GFP, dsRed, CY5 and NIR. There are 4x, 10x, 20x and 40x extra long working distance phase contrast objectives for imaging through plastic dishes.  There is also a 60x, 0.95na objective for high resolution imaging. The scope is equipped with a motorized x/y/z stage and for making panaramic images of slides and plates in both brightfield and fluorescence modes.

Please contact UIC staff in advance of using this system for FURA or NIR imaging as there are hardware adjustments that need to be made.

 

Attention: This instrument is located in 1-220A CCRB

Room 1-220A CCRB
Reserve Equipment
,

Nikon Inverted TiE Deconvolution Microscope System with Ibidi Live Cell Environmental Chamber

Nikon TiE Automated Live Cell Inverted Microscope

Nikon Ti-E Motorized Microscope: Features include Nikon’s patented PFS Perfect Focus III focus lock technology. 100 Watt Illumination system for transmitted and LED reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 40x NAMC Contrast, 60x, and 100x DIC magnifications. Fluorescence accessories for widefield applications for DAPI, GFP, CFP, YFP, RFP and Cy5...and many others. Note: the 20x and 40x objective have correction collars that are adjusted for each user's plate or coverslip thickness.

Off-line Elements Deconvolutionand Workstation:  Elements software and an offline computer just outside the scope room in CCRB in available. It has Elements Deconvolution LIM2D/3D module complete the system and provide a customizable user interface to run deconvolution on entire batches of files making it extremely powerful and easy to use.

The 20x and 40x objectives on this scope can be adjusted for use with various thickness sample plates and slides.  Details on how to adjust these objectives with correction collars can be found here: http://www.microscopyu.com/tutorials/java/aberrations/correctioncollar/i...

Fluorescence Illumination: A Lumencor SpectraX pulsed light engine provides excitation with minimize photobleaching/phototoxicity.

 

 Ibidi Hit Environmental Stage with CO2 and O2 regulation.

http://ibidi.com/xtproducts/en/Instruments-Accessories/Heating-Incubatio...

 

We also have the Braintree Scientific Automated Syringe pump to perfuse solutions! Holds 6 syringes up to 50cc.

http://www.braintreesci.com/prodinfo.asp?number=BS-9000

 
 

 

Room 1-220G CCRB
Reserve Equipment
, ,

Nikon NiE C2 Upright Confocal and Widefield Imaging System

 

Nikon C2 Automated Upright Widefield and Confocal Microscope

Nikon Ni-E Motorized Microscope: Features include Nikon’s newest motorized Ni microscope body. The newly developed CFI Plan Apochromat λ (lambda) series objectives are the key to the series’ optical performance. Nano Crystal Coat, with its ultra low refractive index, provides brighter, high-resolution and high-contrast microscopy images. The CFI 60 objectives for the Ni system are 10x, 20x, 40x oil, and 60x oil, all with DIC capabilities. An LED illumination system for fluorescence, and filters for widefield using DAPI, GFP, and Tex Red filters. The Ni stand uses Nikon’s proprietary stratum structure, used and highly acclaimed for inverted research microscopes, is now incorporated in an upright microscope, allowing a combination of components to be installed. There is a Prior motorized x/y stage and color camera for tiling of large areas of interest in brighfield, DIC, fluorescence and confocal modes.

  • There are 405nm, 488nm, 561nm and 640nm laser lines for excitation.
  • Detectors include are three (3) photo-multiplier tubes (PMTs), plus a transmitted detector.
  • Color digital camera for widefield image capture, including tiling.
  • Includes Elements modules for 2D and 3D Tracking and Measurements, Ratio and FRET imaging.

 

 

Room 1-220B CCRB
Reserve Equipment

Objectives

Please click "Equipment Specs" for a list of our Objectives.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Olympus FluoView FV1000 BX2 Upright Confocal

Olympus FluoView FV1000 is designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging, with minimal risk of damage to living specimens. The system has four fluorescence detectors plus a transmitted light detector for DIC. 

Room 1-162 Jackson Hall
Reserve Equipment

Olympus FluoView FV1000 IX2 Inverted Confocal with FLIM detector

This system is equipped with pulsed 405 and 485 nm lasers for FLIM. FLIM is the acronym for "Fluorescence Lifetime Imaging Microscopy" and is the technique that maps the spatial distribution of fluorescence lifetimes within microscopic images of fixed or living cells. The fluorescence lifetime measures the time that a population of fluorescent molecules spends in the excited state. The fluorescence lifetime does not change upon intensity variations and therefore lifetime measurements are not dependent on the local concentration of fluorophores, or variations in the optical path of the microscope, the local excitation light intensity, or on the local fluorescence detection efficiency.

The FLIM is a time domain system, and uses pulsed lasers at 405 and 488 nm. The detector on the system is a Hybrid GaAsP cathode with three possible emission filter setups - a 480/30nm band pass for CFP emission, a 460LP and a 525/50 nm bandpass filter for GFP. 

 

Room 1-158 Jackson Hall
Reserve Equipment

Olympus IX70 Inverted Fluorescence Microscope

 

The Olympus IX70 Inverted Fluorescence Microscope is a research-level instrument capable of imaging specimens in a variety of illumination modes including brightfield, phase contrast and fluorescence. The scope is equipped with a Diagnostic Instrument 4MP cooled Spot Insight camera which is well suited for both brightfield and low light applications.

Temperature regulation of the stage from ambient to 50 degrees Celsius is possible using the Bioptechs stage and objective temperature controllers for live-cell time-lapse observation.

Room 23A Snyder Hall
Reserve Equipment

Olympus IX70 Inverted Microscope & PixCell

Olympus IX70 inverted fluorescence microscope with Arcturus PixCell II Laser capture micro dissection. The Arcturus CellPix II Laser Capture Microdissection microscope is configured to isolate specific cells of interest from microscopic regions of tissue/cells/organisms for later molecular analysis.

Room 1-161 Jackson Hall
Reserve Equipment

ONIX Microfluidic Perfusion Platform for Live Cell Microscopy

The ONIX Microfluidic Perfusion Platform delivers unprecedented control for live cell imaging experiments. This system is typically associated with our Zeiss Spinning Disk Live Cell Confocal Microscope. The system can all be used on the existing inverted microscope system in your lab to enable dynamic time-lapse experiments. See UIC staff for details.

Room 1-159 Jackson Hall
Reserve Equipment

Panasonic Lumix Cameras

Panasonic Lumix Cameras DMC-FX3

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Panasonic PT-LC76U (Homer)

Conduct your presentations with panache and finesse, using the Panasonic PT-LC76U digital multimedia projector. With its 1600 ANSI Lumens brightness, this Panasonic 3LCD projector offers a clear view of all your projected content, even from a great distance. This digital multimedia projector can project from a distance of up to 1.8 meters - 9.2 meters, enabling you to be non-fussy about its placement.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Panasonic PT-LC76U (Molly)

Conduct your presentations with panache and finesse, using the Panasonic PT-LC76U digital multimedia projector. With its 1600 ANSI Lumens brightness, this Panasonic 3LCD projector offers a clear view of all your projected content, even from a great distance. This digital multimedia projector can project from a distance of up to 1.8 meters - 9.2 meters, enabling you to be non-fussy about its placement.

 

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Panoptiq Digital Slide Imaging System

 

Panoptiq

Panoptiqis an interactive digital imaging system for the dynamic, real-time creation of panoramic microscope images. Unlike WSI scanners, which stitch images in a predefined pattern until completion, Panoptiqallows the user to retain control of image creation and seamlessly stitch together multiple fields of view while scanning the sample using the microscope stage. A high-frame-rate CCD camera attached directly to the user's microscope captures and transmits images to a high-performance computer, where they are processed by intuitive Panoptiqsoftware.

Observe through the Z-axis

The Panoptiq™ Z module permits multi-focal and multi-objective slide review at regions of interest by allowing the user to capture Z-stacks and integrate them into digitally scanned slides. Contrary to conventional Z-stacking, which captures images at confined depth intervals, users of the add-on Z Module can rapidly capture the full range of focal depth as a high-frame-rate video with great precision. This extra Z dimension allows users to visualize the three dimensionality of structures and objects at different focal planes with respect to the slide environment.

Refocus the Image

Detect an out-of-focus area in the panoramic image? Just return to that portion of the slide and readjust the focus with your microscope - the software automatically detects a better focus and replaces the out-of-focus portion. The Panoptiq™ system preserves the best focus achieved on the panoramic image, even if the slide is observed again at a sub-optimal focus.

Room 1-153 Jackson Hall
Reserve Equipment

Pelco BioWave Pro Processor

Sample preparation methododology for light and electron microscopy for live and fixed cell and tissue imaging. 

http://www.tedpella.com/microwave_html/BioWave_Pro.htm

The unit has:

  • Variable power
  • ColdSpot with temperature controlled chiller
  • Vacuum chamber

 

Room 1-220 CCRB
Reserve Equipment

Phillips CM12 Transmission Electron Microscope

Philips CM12 Transmission Electron Microscope (TEM) with motor driven stage and SIA L3C Digital Camera. Includes EDAX Power MX ultra-thin window X-ray microanalysis system, electron diffraction system, super-VHS videotape with audio dub, Ethernet.

Room 37B Snyder Hall
Reserve Equipment

Powerbook G4 (Disco Stu)

Powerbook G4

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Siemens Inveon µPET/CT

 

Interested in imaging using the µPET/CT? please contact the following UIC Staff.

Yung Kim - kim00217@umn.edu

Mark Sanders - msanders@umn.edu

Jason Mitchell - mitch490@umn.edu

Instrument Overview:

A Siemens Inveon preclinical microPET/CT now augments the center's extensive MR capabilities. microPET/CT is a molecular level functional imaging modality that provides valuable insight into biochemical, physiological, pathological and/or pharmacological processes in vivo. The microPET/CT can be used in a number of ways. This information can be obtained via a non-invasive, in vivo imaging process that allows both serial and longitudinal studies to be performed and in a continuous and comparative way. Other areas can be assessed such as novel drug delivery, effects of intervention, and progression and outcome of a particular therapy. Examples of biomedical applications specific to the microPET/CT using FDG:

  • Myocardial viability.
  • Tumor glucose metabolism.
  • Gastrointestinal graft-versus-host disease (GVHD).
  • Neurotransmitter function in vivo over time, e.g. Huntington's, Parkinson's, Alzheimer's disease.
  • Time-activity profiles reflecting tracer delivery, binding and washout, i.e., pharmokinetics.
  • Development of new radiopharmaceuticals for use in diagnostic imaging and new molecular imaging assays.
  • Acquisition of sequential PET and CT data without repositioning.
Room 1-164 CCIR (Contact UIC Staff)
Reserve Equipment

Small Animal Image Processing Computer

The Small Animal Image Processing Computer is loaded with the following analysis software:
 
Bruker Extreme
IVIS Living Image
Vevo 2100 Ultrasound
FIJI
Microsoft Office
Adobe Acrobat Reader
FireFox
Antie Virus
 
The computer is mapped to our nas server for temporary data storage.
Room 1-250B CCRB
Reserve Equipment

Sony DCR-TRV900 Digital Video Camera

The Sony DCR-TRV900 can handle up to eight hours recording. The 12X optical zoom lets you move in or pull back for wide shots, while the precision 48X digital zoom uses a hyper-precision CCD to maintain superior image detail.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Storm 860 Molecular Imager

The Storm 860 Molecular Imager Gel and Blot Imaging System offers filmless autoradiography, fluorescence and chemifluorescence imaging. Equipped with 450 nm  and 635 nm excitation lasers.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

The Electron Microscopy Transfer Server

Transfer server connection instructions:

OSX (MAC): From Finder select "Go" in the menu bar then select "Connect to Server.." in the drop down menu. Enter the server address, smb://160.94.173.214/share1 , and click connect. Connect as a Registered User with:
Name: transfer and Password: transfer
Windows (PC): From Windows Explorer select "Tools" from the menu bar then select "Map network device..." in the drop down menu. Enter the server address, \\160.94.173.214\share1 , and click Finish. Connect to the server using :
Username: transfer and the Password: transfer
Room 23A Snyder Hall
Reserve Equipment

Toshiba Tablet PC (Sedusa)

Toshiba Tablet PC

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Tousimis Autosamdri-814 Critical Point Dryer

Critical Point Dryer

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Typhoon FLA 9500

Typhoon FLA 9500 is a variable mode laser scanner with modular access to the optical components, providing both versatile and flexible imaging for precise quantitation of proteins, nucleic acids, and other biomolecules.

1-151 Jackson Hall (Minneapolis Campus)
Reserve Equipment

Vevo 2100 System (CCRB)

The first high-frequency, high-resolution digital imaging platform with linear array technology and color doppler mode.

Instrument details can be found here: http://www.visualsonics.com/vevo2100#

Room 1-260D CCRB
Reserve Equipment

Vevo 2100 System (JH)

The first high-frequency, high-resolution digital imaging platform with linear array technology and color doppler mode.
Detailed specifications can be found here:  http://www.visualsonics.com/vevo2100

Room 1-166A Jackson Hall
Reserve Equipment

Vixia HF M31 High Def Video Camera (Kang)

The Canon VIXIA HF M31 Dual Flash Memory Camcorder features a 32GB internal flash drive and an SD memory card slot in an ultra-sleek, compact and lightweight body - it's a brilliant blend of capability and convenience. Canon's advanced imaging technologies, including a Genuine Canon 15x HD Video Lens and 3.89-megapixel Full HD CMOS Image Sensor, help to deliver high definition video and 3.3 megapixel photos with incredible detail and lifelike color. Sophisticated features such as Smart Auto "reads" the visual components of the scene you're shooting and chooses the best settings. Touch & Track allows you to achieve sharp focus and precise exposure for any subject, simply by touching the 2.7" Touch Panel LCD. And to complete the entertainment picture, 5.1-Channel Surround Sound* ensures your audio is every bit as impressive as your video.

23 Snyder Hall (Saint Paul Campus)
Reserve Equipment

Xenogen IVIS 100 in vivo Imaging System

ATTENTION: THIS SYSTEM IS LOCATED IN THE CANCER CARDIOVASCULAR RESEARCH BUILDING, RM 1-260C.

The IVIS 100 Imaging System is a highly sensitive and large field of view imaging system that offers users the flexibility to image bioluminescent reporters both in vivo and in vitro. It relies on patented optical imaging technologies to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

•Please do not use this equipment without contacting UIC staff for training and approval. Request training here:

  http://uic.umn.edu/node/add/training-request

•Please accurately reserve and record all usage on the appropriate reservation calendar. Reserve and record usage here:

  http://uic.umn.edu/content/uic-reservation-system

•Please return all animals to the Catch Room (rm 1-370F) following imaging.  Contact RAR staff for housing information.

•Please contact UIC staff with any questions.

  http://uic.umn.edu/

 

Room 1-260C CCRB
Reserve Equipment
,

Zeiss Axioplan 2 Upright Microscope

Brightfield, Fluorescence and DIC.

Has a 12MP color, perfect for H&E stained sections and cooled, perfect for fluorescence.

Motorized x/y stage and focus motor for z-series collection and deconvolution.

Room 1-161 Jackson Hall
Reserve Equipment

Zeiss Cell Observer SD Spinning Disk Confocal

LIVE CELL IMAGING

 

Spinning disk confocal fluorescence microscope for imaging live samples at fast speed. Definite focus, time-lapse, multi-well and tiling of images. DIC and phase contrast capable. Full environmental chamber for temperature and atmosphere contol. Three laser lines available (405, 488, 561 nm). 

Room 1-160 Jackson Hall
Reserve Equipment

Zeiss TIRF Scope

The TIRF imaging system achieves the best contrast and optimum resolution in x, y and z. It permits examinations of layer thicknesses of < 200 nm as well as examinations of processes at the cell membrane and in the cell-free space, in a very thin layer and in real time. 

The system provides full environmental control and allows long term time lapse in epifluorescence, TIRF, phase contrast and DIC. 

 

LIVE CELL IMAGING

Room 1-164 Jackson Hall
Reserve Equipment