University of Minnesota
University Imaging Center
http://uic.umn.edu/
612.624.7938
University Imaging Centers - Where the University sees the future

Equipment

Equipment Description Location

Nikon A1 Spectral Confocal Microscope

The Nikon A1si is a confocal system equipped with a point-scan scan head, 5 standard PMT detectors and a 32-channel PMT spectral detector. The A1si's highest resolution mode is up to 4096 x 4096 pixels. The system is mounted on a Nikon Ti2000E inverted, fluorescence microscope with DIC optics. NIS Elements imaging software is used to control acquisition and analysis.

Advantages of multispectral imaging and unmixing has been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging. 

A few example citations are:

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging "Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct;Chapter 14:Unit 14.19. doi: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.

Room 35D Snyder Hall

Nikon A1R FLIM Confocal Microscope

LIVE CELL IMAGING

Nikon Ti-E Motorized Microscope: Features include Nikon's patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 20x water immersion, 40x oil, and 60x oil magnifications for DIC. Fluorescence illumination and filters for widefield applications for DAPI, CFP, GFP, YFP, RFP, and Cy5. The scope is equipped for widefield imaging using a Hamamatsu Flash 4 V3 CMOS camera.

  • A1R GaAsP Confocal System
  • Laser Selection: 405nm, 445nm, 488nm, 514nm, 561nm and 640nm.
  • AR1 Confocal Scanner: Nikon's exclusive hybrid scanner allows flexible switching or simultaneous use of two galvo scanners (resonant and non-resonant) this allows ultrafast imaging (up to 420fps) and photo activation imaging required to unveil cell dynamics and interaction.
  • Detectors: Included are 4 confocal detectors (2 GaSaP) plus transmitted detector.

The Picoquant Fluorescence Lifetime Imaging (FLIM) system has been installed and is operational.  It has 405, 485 and 560 nm pulsed laser and cand do many FLIM and FCS techniques. Please contact UIC staff for training.

Learn more about FLIM here: http://tcspc.com/doku.php  ath the 

Time Correlated Single Photon Counting Wiki

 

 

Room 1-220H CCRB
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Nikon A1Rsi Confocal w/ SIM Super Resolution

LIVE CELL IMAGING

 

Nikon Ti-E Motorized Microscope: Features include Nikon’s patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 10x, 20x, 40x, 60x, and 100x magnifications for DIC. Fluorescence accessories for widefield applications for DAPI, GFP, RFP, and Cy5.

·      A1R Confocal System

·      Laser Selection

405nm, 488nm, 561nm and 640nm

·       A1R Scanner- Nikon's exclusive hybrid scanner allows flexible switching or simultaneous use of two galvo scanners (resonant and non-resonant) this allows ultrafast imaging and photo activation imaging required to unveil cell dynamics and interaction.

·      Detectors- Included are 4 channel GaSP detectors, transmitted, and 32 channel Si (Spectral) detectors.

·      SIM Structured Illumination System-Using high frequency Structured Illumination, the Nikon N-SIM can achieve image resolution of 85nm*, which was previously considered impossible with optical microscopes. Furthermore, with temporal resolution of up to 0.6 sec/frame**, N-SIM enables super resolution time-lapse imaging capture of dynamic molecular interactions in living cells. Learn more about the product here: http://www.nikoninstruments.com/Products/Microscope-Systems/Inverted-Microscopes/N-SIM-Super-Resolution

·      Laser Selection

405nm, 488nm, 561nm and 640nm

·      A1/SIM Camera, Software and Workstation

Elements software and an Andor iXon camera complete the system and provide an easy to use customizable user interface to make the Nikon A1R/SIM system a turnkey live cell imaging system with multiple capabilities. 

Room 1-159 Jackson Hall
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Nikon AZ100 C1si Spectral Confocal Macroscope

New confocal imaging system for high-definition macro acquisition.

Our AZ100 Multizoom Macroscope with the C1si Spectral Confocal attachment, make the ultimate platform for in vivo imaging. Ideal for plant biology, developmental biology, cell biology, stem cell and tissue research, the AZ-C1si allows the researcher to view large specimens in confocal mode. The AZ-C1si can not only capture fields of view up to 1cm and permits deeper confocal imaging than conventional microscopes thanks to its large working distance objectives. Whole organisms can be monitored and documented over time (for example, embryos) offering a wealth of continuous information on development or the organism’s response to experimental variables. Both conventional PMTs and Spectral detectors are installed on this instrument.

The system is equipped with 1x, 4x and 5x objectives with variable optical zoom.  There are 405, 457, 476, 488, 514, 561, 637 nm lasers for image, co-localization, FRAP, FRET and spectral studies.

The system is run using Nikon Elements Software.

Advantages of multispectral imaging and unmixing has been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging. 

A few example citations are:

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging "Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct;Chapter 14:Unit 14.19. doi: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.

23 Snyder Hall (Saint Paul Campus)
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Nikon NiE C2 Upright Confocal and Widefield Imaging System

 

Nikon C2 Automated Upright Widefield and Confocal Microscope

Nikon Ni-E Motorized Microscope: Features include Nikon’s newest motorized Ni microscope body. The newly developed CFI Plan Apochromat λ (lambda) series objectives are the key to the series’ optical performance. Nano Crystal Coat, with its ultra low refractive index, provides brighter, high-resolution and high-contrast microscopy images. The CFI 60 objectives for the Ni system are 10x, 20x, 40x oil, and 60x oil, all with DIC capabilities. An LED illumination system for fluorescence, and filters for widefield using DAPI, GFP, and Tex Red filters. The Ni stand uses Nikon’s proprietary stratum structure, used and highly acclaimed for inverted research microscopes, is now incorporated in an upright microscope, allowing a combination of components to be installed. There is a Prior motorized x/y stage and color camera for tiling of large areas of interest in brighfield, DIC, fluorescence and confocal modes.

  • There are 405nm, 488nm, 561nm and 640nm laser lines for excitation.
  • Detectors include are three (3) photo-multiplier tubes (PMTs), plus a transmitted detector.
  • Color digital camera for widefield image capture, including tiling.
  • Includes Elements modules for 2D and 3D Tracking and Measurements, Ratio and FRET imaging.

 

 

Room 1-220B CCRB
Objectives

Please click "Equipment Specs" for a list of our Objectives.

23 Snyder Hall (Saint Paul Campus)

Olympus FluoView FV1000 BX2 Upright Confocal

Olympus FluoView FV1000 is designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging, with minimal risk of damage to living specimens. The system has four fluorescence detectors plus a transmitted light detector for DIC. 

Room 1-162 Jackson Hall

Olympus FluoView FV1000 IX2 Inverted Confocal with FLIM detector

This system is equipped with pulsed 405 and 485 nm lasers for FLIM. FLIM is the acronym for "Fluorescence Lifetime Imaging Microscopy" and is the technique that maps the spatial distribution of fluorescence lifetimes within microscopic images of fixed or living cells. The fluorescence lifetime measures the time that a population of fluorescent molecules spends in the excited state. The fluorescence lifetime does not change upon intensity variations and therefore lifetime measurements are not dependent on the local concentration of fluorophores, or variations in the optical path of the microscope, the local excitation light intensity, or on the local fluorescence detection efficiency.

The FLIM is a time domain system, and uses pulsed lasers at 405 and 488 nm. The detector on the system is a Hybrid GaAsP cathode with three possible emission filter setups - a 480/30nm band pass for CFP emission, a 460LP and a 525/50 nm bandpass filter for GFP. 

 

Room 1-158 Jackson Hall

Zeiss Cell Observer SD Spinning Disk Confocal

LIVE CELL IMAGING

 

Spinning disk confocal fluorescence microscope for imaging live samples at fast speed. Definite focus, time-lapse, multi-well and tiling of images. DIC and phase contrast capable. Full environmental chamber for temperature and atmosphere contol. Three laser lines available (405, 488, 561 nm). 

Room 1-160 Jackson Hall