University of Minnesota
University Imaging Center
http://uic.umn.edu/
612.624.7938
University Imaging Centers - Where the University sees the future

Equipment

Equipment Description Location

Leica MZ FL III Fluorescence Stereomicroscope

Sort and manipulate and image your specimens with the stereo objective in 3D, with large fields of view, large working distances and an outstanding depth of field. Simply switch to the HR (High Resolution) micro-objective and analyze the quality of the gene expression immediately and without delay via an optical light microscope at a resolution of 0.7 µm (1320 pairs of lines/mm) and an 800x magnification.

Room 1-250D CCRB
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LMD6500 Laser Dissection Microscope
Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected for subsequent molecular analysis.

Researchers can selectively and routinely analyze regions of interest down to single cells from all kinds of tissues (cryo or paraffin sections 4-20 µm thick), even living cells from cell culture.  

Check out this training video using the LMD6500 on JOVE: http://www.jove.com/video/51168/production-rna-for-transcriptomic-analys...

You have the abiltiy to de-paraffinize your slides on site.

LDM works best when using PEN membrane slides.  The membrane only fram slides are available in the LifeTechnolgy Supply Center on site or you can order them here: http://www.lifetechnologies.com/order/catalog/product/LCM0521?ICID=searc...

The PEN membrane on glass slides are here:https://www.thermofisher.com/order/catalog/product/LCM0522?ICID=search-p...

 

Videos on the LDM technique can be found here: http://www.thermofisher.com/us/en/home/life-science/gene-expression-anal...

Room 1-220B CCRB
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Nikon AZ100M Macro Fluorescence Scope

 

Nikon AZ100 Motorized Fluorescence Macroscope

  • Fluorescence (UV, CFP, GFP, YFP, dsRed and CY5 fliter sets ) and transmitted light with DIC
  • 1x and 4x PlanApo objectives
  • 1X to 8X Optical Zoom Range
  • Photometrics ES2 greyscale 12-bit camera for fluorescence
  • Nikon DS-Ri1 color 12-bit camera up to 4076x3116 pixels for full color imaging
  • Motorized X/Y stage for multi-position or panoramic images
  • Motorized Z for z-series collections for 3D and Extended Depth of Field Imaging
  • Nikon Elements AR software for multi-dimentional data collection

 

Room 1-161 Jackson Hall

Nikon BioStation IM Live Cell Incubator and Imaging in Jackson and CCRB locations

LIVE CELL IMAGING

The Nikon BioStation IM is a compact live cell incubator and imaging system that allows users to conduct live cell imaging without a steep learning curve. The BioStation IM facilitates long term time-lapse experiments, including studies of cell growth, morphology, and protein expression, by providing consistent environmental control of temperature, humidity and gas concentration in combination with phase and fluorescence imaging (blue and green excitation) of exceptional quality. Multiple postions and z-series can be collected at various magnifications from 20x to 80x (0.8na). Additional fluorescence filters sets for UV, CYP, YFP and CY5 are available, with any two possible in each experiment.

There are Nikon Biostations located in both our Jackson Hall and CCRB locations.

The systems are best used with polymer or glass-bottomed 35mm dishes. 

Inserts are available to allow for multiple samples per experiment.

 

Learn more about best media and approaches for live cell imaging here: https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellu...

 

Room 1-161 Jackson Hall

Nikon Eclipse 90i

 

The Nikon Eclipse 90i is a fully motorized upright microscope configured to image in transmitted light, DIC and epi-fluorescence modes. Fluorescence cubes available for DAPI, GFP, RFP, and HYQ Cy5. Fluorescence illumination is provided by a Lumencor Sola Light Engine. You can do timelapse, XYZ position, Z stacks, multiple excitation or emission wavelengths, and tiling experiments. Equipped with a Hamamatsu Orca Flash 4.0 v2 CMOS monochrome camera and a Nikon D2-Fi2 color camera running on Nikon Elements software.

Room 23C Snyder Hall
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Nikon Eclipse TE2000-U Inverted Microscope

Nikon TE2000  Inverted Microscope

 

This Nikon TE2000 inverted microscope is equipped with brightfield, phase contrast and fluorescence capabilities.  It has a high sensitivity Nikon Ds-Fi1 color camera  (2560x1920 pixels) operated using Elements software.  

Fluorescence illumination is provided by a SOLA light engine or a Xenon Light source and the scope has filter sets for FURA, UV, CFP, GFP, dsRed, CY5 and NIR. There are 4x, 10x, 20x and 40x extra long working distance phase contrast objectives for imaging through plastic dishes.  There is also a 60x, 0.95na objective for high resolution imaging. The scope is equipped with a motorized x/y/z stage and for making panaramic images of slides and plates in both brightfield and fluorescence modes.

Please contact UIC staff in advance of using this system for FURA or NIR imaging as there are hardware adjustments that need to be made.

 

Attention: This instrument is located in 1-220A CCRB

Room 1-220A CCRB
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Nikon Inverted TiE Deconvolution Microscope System with Ibidi Live Cell Environmental Chamber

Nikon TiE Automated Live Cell Inverted Microscope

Nikon Ti-E Motorized Microscope: Features include Nikon’s patented PFS Perfect Focus III focus lock technology. 100 Watt Illumination system for transmitted and LED reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 40x NAMC Contrast, 60x, and 100x DIC magnifications. Fluorescence accessories for widefield applications for DAPI, GFP, CFP, YFP, RFP and Cy5...and many others. Note: the 20x and 40x objective have correction collars that are adjusted for each user's plate or coverslip thickness.

Off-line Elements Deconvolutionand Workstation:  Elements software and an offline computer just outside the scope room in CCRB in available. It has Elements Deconvolution LIM2D/3D module complete the system and provide a customizable user interface to run deconvolution on entire batches of files making it extremely powerful and easy to use.

The 20x and 40x objectives on this scope can be adjusted for use with various thickness sample plates and slides.  Details on how to adjust these objectives with correction collars can be found here: http://www.microscopyu.com/tutorials/java/aberrations/correctioncollar/i...

Fluorescence Illumination: A Lumencor SpectraX pulsed light engine provides excitation with minimize photobleaching/phototoxicity.

 

Learn more about live cell imaging reagents here:https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellu...

 

 Ibidi Environmental Stage with CO2 and O2 regulation:

https://ibidi.com/66-heating-systems-for-slides-dishes

Dishes and chambered slides can be found here:

https://ibidi.com/12-labware

We also have the Braintree Scientific Automated Syringe pump to perfuse solutions! Holds 6 syringes up to 50cc.

http://www.braintreesci.com/prodinfo.asp?number=BS-9000

 
 

 

Room 1-220G CCRB

Olympus IX70 Inverted Fluorescence Microscope

 

The Olympus IX70 Inverted Fluorescence Microscope is a research-level instrument capable of imaging specimens in a variety of illumination modes including brightfield, phase contrast and fluorescence. The scope is equipped with a Diagnostic Instrument 4MP cooled Spot Insight camera which is well suited for both brightfield and low light applications.

Temperature regulation of the stage from ambient to 50 degrees Celsius is possible using the Bioptechs stage and objective temperature controllers for live-cell time-lapse observation.

Room 23A Snyder Hall

Olympus IX70 Inverted Microscope & PixCell

Olympus IX70 inverted fluorescence microscope with Arcturus PixCell II Laser capture micro dissection. The Arcturus CellPix II Laser Capture Microdissection microscope is configured to isolate specific cells of interest from microscopic regions of tissue/cells/organisms for later molecular analysis.

Room 1-161 Jackson Hall
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Zeiss Axioplan 2 Upright Microscope

Brightfield, Fluorescence and DIC.

Has a 12MP color, perfect for H&E stained sections and cooled, perfect for fluorescence.

Motorized x/y stage and focus motor for z-series collection and deconvolution.

Room 1-161 Jackson Hall

Zeiss TIRF Scope

The TIRF imaging system achieves the best contrast and optimum resolution in x, y and z. It permits examinations of layer thicknesses of < 200 nm as well as examinations of processes at the cell membrane and in the cell-free space, in a very thin layer and in real time. 

The system provides full environmental control and allows long term time lapse in epifluorescence, TIRF, phase contrast and DIC. 

 

LIVE CELL IMAGING

Room 1-164 Jackson Hall