University of Minnesota
University Imaging Center
http://uic.umn.edu/
612.624.7938
University Imaging Centers - Where the University sees the future

Equipment

Equipment Description Location

Countess Automated Cell Counter

The Countess® Automated Cell Counter offers easy and accurate cell counting and viability counts. You can get all the data you need about your cell cultures in just 30 seconds without using a hemocytometer.

Room 1-220E CCRB

Cressington 108 Auto Sputter Coater
The Cressington 108 Auto Sputter Coater deposits a thin layer of metal (60% gold / 40% paladium) on mounted samples for improved signal in the SEM.  The coater is equipped with a rotating-tilting stage for better sample coverage, and a thickness detector for controlled metal deposition.
23 Snyder Hall (Saint Paul Campus)

Denton DV-502 Vacuum Evaporator

The Denton Vacuum Evaporator is used in many different specimen preparation methods — mostly employed in electron imaging. Thicker coatings of metal (such as gold, platinum:palladium, or gold:palladium) can be applied to specimens of nearly any shape.

23 Snyder Hall (Saint Paul Campus)

Leica AFS2 Freeze Substitution and Low Temperature Embedding System
The Leica EM AFS2 performs freeze substitution and progressive lowering temperature (PLT) techniques and allows low temperature embedding and polymerization of resins.
Room 37A Snyder Hall

Leica CM1800 Cryostat

Leica CM 1800 Cryostat utilizes up-to date microprocessor technology ensuring easy operation. All functions are protected against dirt and humidity by a plastic foil. It features an easily readable display including an automatic display of errors and a motorized coarse feed which enables rapid trimming and sectioning.

Room 37A Snyder Hall

Leica EM Tissue Processor

Tissues can be processed into a variety of resins for subsequent electron and light microscopy analysis. The pre-heat/cool system allows the correct reagent temperature to be maintained throughout processing. Small vials are available for EM processing and larger for LM which can also be used for high throughput sample processing runs where over 150 EM samples can be processed in one run.

23 Snyder Hall (Saint Paul Campus)

Leica Ultracut UCT Ultramicrotome

The ultramicrotome is an instrument commonly used to prepare TEM samples. It uses either a glass or diamond knife to cut ultra thin sections of specimens embedded in epoxy. The sections are approximately 60 to 100 nanometers in thickness and 0.5 to 3 mm in width and can be stained and mounted onto TEM grids for viewing. The ultramicrotome is also used to create mirror-smooth surfaces on samples mounted in epoxy, the applications for which include secondary imaging on the SEM and nanoindentation. Preparation on smooth samples for surface analysis need not always be of samples that are embedded, as sometimes bulk will work.

23 Snyder Hall (Saint Paul Campus)

Pelco BioWave Pro Processor

Sample preparation methododology for light and electron microscopy for live and fixed cell and tissue imaging. 

http://www.tedpella.com/microwave_html/BioWave_Pro.htm

The unit has:

  • Variable power
  • ColdSpot with temperature controlled chiller
  • Vacuum chamber

 

Room 1-220 CCRB

Tousimis Autosamdri-814 Critical Point Dryer

Critical Point Dryer

23 Snyder Hall (Saint Paul Campus)