University of Minnesota
University Imaging Center
http://uic.umn.edu/
612.624.7938
University Imaging Centers - Where the University sees the future

Equipment

Equipment Description Location

Countess Automated Cell Counter

The Countess® Automated Cell Counter offers easy and accurate cell counting and viability counts. You can get all the data you need about your cell cultures in just 30 seconds without using a hemocytometer.

Room 1-220E CCRB

Cressington 108 Auto Sputter Coater
The Cressington 108 Auto Sputter Coater deposits a thin layer of metal (60% gold / 40% paladium) on mounted samples for improved signal in the SEM.  The coater is equipped with a rotating-tilting stage for better sample coverage, and a thickness detector for controlled metal deposition.
23 Snyder Hall (Saint Paul Campus)

Denton DV-502 Vacuum Evaporator

The Denton Vacuum Evaporator is used in many different specimen preparation methods — mostly employed in electron imaging. Thicker coatings of metal (such as gold, platinum:palladium, or gold:palladium) can be applied to specimens of nearly any shape.

23 Snyder Hall (Saint Paul Campus)

Leica AFS2 Freeze Substitution and Low Temperature Embedding System
The Leica EM AFS2 performs freeze substitution and progressive lowering temperature (PLT) techniques and allows low temperature embedding and polymerization of resins.
Room 37A Snyder Hall

Leica CM1800 Cryostat

Leica CM 1800 Cryostat utilizes up-to date microprocessor technology ensuring easy operation. All functions are protected against dirt and humidity by a plastic foil. It features an easily readable display including an automatic display of errors and a motorized coarse feed which enables rapid trimming and sectioning.

Room 37A Snyder Hall

Leica EM Tissue Processor

Tissues can be processed into a variety of resins for subsequent electron and light microscopy analysis. The pre-heat/cool system allows the correct reagent temperature to be maintained throughout processing. Small vials are available for EM processing and larger for LM which can also be used for high throughput sample processing runs where over 150 EM samples can be processed in one run.

23 Snyder Hall (Saint Paul Campus)

Leica Ultracut UCT Ultramicrotome

The ultramicrotome is an instrument commonly used to prepare TEM samples. It uses either a glass or diamond knife to cut ultra thin sections of specimens embedded in epoxy. The sections are approximately 60 to 100 nanometers in thickness and 0.5 to 3 mm in width and can be stained and mounted onto TEM grids for viewing. The ultramicrotome is also used to create mirror-smooth surfaces on samples mounted in epoxy, the applications for which include secondary imaging on the SEM and nanoindentation. Preparation on smooth samples for surface analysis need not always be of samples that are embedded, as sometimes bulk will work.

23 Snyder Hall (Saint Paul Campus)

Pelco BioWave Pro Processor

Sample preparation methododology for light and electron microscopy for live and fixed cell and tissue imaging. 

http://www.tedpella.com/microwave_html/BioWave_Pro.htm

The unit has:

  • Variable power
  • ColdSpot with temperature controlled chiller
  • Vacuum chamber

 

Room 1-220 CCRB

PELCO easiSlicer Vibratome

The PELCO vibratome provides exceptional stability and perfect control of section thickness down to 40 microns, with minimal z-axis vibration.

  • Precision design for accurate, high quality sections with minimal z-axis vibration
  • Novel bath design utilizes unique easy-locking base making it simple to clean
  • Anodized specimen tray magnetically locks onto bath
  • Optional magnetic base for tissue mounts available
  • Cuts sections to 40 microns
  • LED lights indicate active vibratory status and cutting window boundaries
  • Standard removable blade holder securely holds Feather® blades at a 15º 
    preset angle; other optional angles available
Room 1-250C CCRB

Tousimis Autosamdri-814 Critical Point Dryer

Critical Point Dryer

23 Snyder Hall (Saint Paul Campus)
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X-Clarity

The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to make electrophoretic tissue clearing simple, rapid, and efficient. Examine your samples with a new perspective with the X-CLARITY™. We have had good success with brain, kidney, heart, intestine, skeletal muscle, brown fat, lung, liver and even plant tissues. This technique is well suited for both endogenous fluorescence protein expression retention and many immunological techniques.

  1. Meet with UIC staff to discuss your clearing project
  2. Drop off your fixed tissue and probes if needed
  3. We do the clearing and labeling
  4. We meet to do the imaging

We have ETC sample holders of various sizes including:

Holder for 6 Mouse Brain sized samples
Holder for 2 Rat Brain sized samples
Holder for Mouse Brain Slices (<1.5mm each)
Holder for Rat Brain Slices (<1.5mm each)
Holder for 48 Samples (<3 cubic mm each)

Room 1-220 CCRB