University of Minnesota
University Imaging Center
University Imaging Centers - Where the University sees the future

Nikon A1 Spectral Confocal Microscope

Equipment Type: 
UMN Rate: 
$50.00 Per Hour
PDF icon Confocal Imaging Tips.pdf1.08 MB

The Nikon A1si is a confocal system equipped with a point-scan scan head, 5 standard PMT detectors and a 32-channel PMT spectral detector. The A1si's highest resolution mode is up to 4096 x 4096 pixels. The system is mounted on a Nikon Eclipse Ti2 inverted, fluorescence microscope with DIC optics. NIS Elements imaging software is used to control acquisition and analysis.

Advantages of multispectral imaging and unmixing has been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging. 

A few example citations are:

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging "Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct;Chapter 14:Unit 14.19. doi: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.