University of Minnesota
University Imaging Center
http://uic.umn.edu/
612.624.7938
University Imaging Centers - Where the University sees the future

Nikon A1R FLIM Confocal Microscope

Equipment Type: 
Status: 
Active
Location: 
UMN Rate: 
$50.00 Per Hour
Capabilities: 

LIVE CELL IMAGING

Do you need: 

  • Six (6) laser lines of excitation?
  • A system fully configured for screening of plates and slides?
  • A modular system for assay development (Nikon Elements and JOBS)?
  • Confocal imaging for 3D samples?
  • Environmental control for live cell imaging?

Nikon Ti-E Motorized Microscope: Features include Nikon's patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 20x water immersion, 40x oil, and 60x oil magnifications for DIC. Fluorescence illumination and filters for widefield applications for DAPI, CFP, GFP, YFP, RFP, and Cy5. The scope is equipped for widefield imaging using a Hamamatsu Flash 4 V3 CMOS camera.

  • A1R GaAsP Confocal System
  • Laser Selection: 405nm, 445nm, 488nm, 514nm, 561nm and 640nm.
  • AR1 Confocal Scanner: Nikon's exclusive hybrid scanner allows flexible switching or simultaneous use of two galvo scanners (resonant and non-resonant) this allows ultrafast imaging (up to 420fps) and photo activation imaging required to unveil cell dynamics and interaction.
  • Detectors: Included are 4 confocal detectors (2 GaSaP) plus transmitted detector.

The Picoquant Fluorescence Lifetime Imaging (FLIM) system has 405, 485 and 560 nm pulsed lasers and cand do many FLIM and FCS techniques. Please contact UIC staff for training.

Learn more about FLIM here: http://tcspc.com/doku.php  ath the 

Time Correlated Single Photon Counting Wiki