University of Minnesota
University Imaging Center
http://uic.umn.edu/
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University Imaging Centers - Where the University sees the future

Nikon AZ100 C1si Spectral Confocal Macroscope

Equipment Type: 
Status: 
Active
UMN Rate: 
$35.00 Per Hour
Manual: 
AttachmentSize
PDF icon Confocal Imaging Tips.pdf1.08 MB
Capabilities: 

New confocal imaging system for high-definition macro acquisition.

Our AZ100 Multizoom Macroscope with the C1si Spectral Confocal attachment, make the ultimate platform for in vivo imaging. Ideal for plant biology, developmental biology, cell biology, stem cell and tissue research, the AZ-C1si allows the researcher to view large specimens in confocal mode. The AZ-C1si can not only capture fields of view up to 1cm and permits deeper confocal imaging than conventional microscopes thanks to its large working distance objectives. Whole organisms can be monitored and documented over time (for example, embryos) offering a wealth of continuous information on development or the organism’s response to experimental variables. Both conventional PMTs and Spectral detectors are installed on this instrument.

The system is equipped with 1x, 4x and 5x objectives with variable optical zoom.  There are 405, 457, 476, 488, 514, 561, 637 nm lasers for image, co-localization, FRAP, FRET and spectral studies.

The system is run using Nikon Elements Software.

Advantages of multispectral imaging and unmixing has been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging. 

A few example citations are:

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging "Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct;Chapter 14:Unit 14.19. doi: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.