University of Minnesota
University Imaging Center
http://uic.umn.edu/
612.624.7938
University Imaging Centers - Where the University sees the future

Announcements

Minnesota Cryo-EM Mini Symposium

The Characterization Facility (CSE), Institute for Molecular Virology, and the Department of Veterinary and Biomedical Sciences are hosting a Minnesota Cryo-EM Mini Symposium.

Speakers:

Dr. Jeffrey Legyel (Thermo Fisher Scientific) - "In situ Structural Biology - Advances In Cryo-electron Microscopy"

Dr. Fang Li (UMN) - "Cryo-electron Microscopic Studies of Coronavirus Spike Proteins"

Dr. Paul Jardine (UMN) - "High Resolution Cryo-EM of Phage Assembly"

  • Date: Friday, December 14, 2018
  • Time: 9:30 a.m. - Noon.
  • Where: 1-520 Moos Tower, East Bank Campus

Click here to download the flyer.

The University Imaging Centers are Hiring

The University Imaging Centers are Hiring!

We are seeking applicants who are service-oriented team players with excellent interpersonal, organizational and communication skills who enjoy moving science forward using state-of-the-art imaging resources.

The successful candidate will join a team of UIC associates responsible for the daily interactions of a dynamic interdisciplinary core facility at a major research University. This candidate will support and advance imaging projects primarily at our Jackson Hall location. This will include working on widefield, confocal, two photon, in vivo imaging and other efforts by the UIC.

We are looking for a degree in biology, chemistry or biomedical imaging. There is a need to be ready to help researchers with both trivial and advanced aspects of imaging projects. Experience in sample preparation (fixation, staining, antibody labeling, etc.), and advanced light microscopy is required and/or some experience with image analysis would be a strong plus. The successful candidate should be prepared to be in a dynamic multi-user environment whereby four basic areas of service must be covered:

  • Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users. 
  • Bring expertise in new imaging technologies to users.
  • Educate the University community about new imaging technologies.
  • Promote interaction between developers of new imaging technology and potential users.

To learn more about the position or apply visit the UMN job posting.

High Resolution Form 2 SLA 3D Printing

 

 

The University Imaging Centers has recently installed a Form 2 SLA 3D printer.

Stereolithography (SLA) 3D printing uses a laser to cure solid isotropic parts from a liquid photopolymer resin delivering high-resolution parts.

Stereolithography is a form of 3D printing technology used for creating models layer by layer using photopolymerization, a process by which light causes chains of molecules to link, forming polymers. Those polymers then make up the body of a three-dimensional solid.

To learn more visit UIC 3D Printing.

Scientific American: Superslow Brain Waves May Play a Critical Role in Consciousness

Credit: Getty Images

Every few seconds a wave of electrical activity travels through the brain, like a large swell moving through the ocean. Scientists first detected these ultraslow undulations decades ago in functional magnetic resonance imaging (fMRI) scanes of people and other animals at rest–but the phenomenon was thought to be either electrical "noise" or the sum of much faster brain signals and was largely ignored.

Now a study that measured these "infraslow" (less than 0.1 hertz) brain waves in mice suggests they are a distinct type of brain activity that depends on an animal's concious state. But big questions remain about these waves' origin and function.

Continue reading at Scientific American.

UIC Update: I Can See Clearly Now...

The University Imaging Centers (UIC) is excited to announce new capabilities to our sample preparation services to include whole tissue clearing and labeling. Biological specimens are intrinsically three-dimensional; however light scattering while imaging deeply into a tissue volume is problematic. Efforts to eliminate the scatter by “clearing” the tissue have been ongoing for well over a century, with a large number of recent innovations, under names such as CLARITY, PACT/PARS, iDISCO, SeeDB, CUBIC, Scale and a host of others.

The subsequent imaging of this cleared tissue is the next challenge and single and multi-photon confocal microscopes and light-sheet instruments are leading the way in resolution and speed of acquisition.

A third challenge is the data. With dataset in the 10s to 1000s of GBs per image the data’s shear volume is a significant challenge.

The UIC is expanding its service offering in all three areas.  1) we will be hiring a full-time staff member to identify the best clearing methodology. We have also optimized clearing methods using microwave-assisted methods developed in the UIC as well as commercial clearing systems (XClarity).  We have posted a position (UIC Clearing Job) to validate and optimize the appropriate clearing methodology for our expansive user base.  2) We have macro and micro-scale objectives specially designed for cleared tissue for our confocal and diSPIM light sheet systems. We are further evaluating additional imaging tools to add to our imaging resources.  3) We have added to our computational hardware and software (OpenSPIM, AMIRA, Imaris and Nikon Elements) These efforts are possible thanks to the joint support of ITN, the MDT addiction team, Department of Neuroscience, the AHC/Medical School, the OVPR’s office and the UIC.